Effect of Low Dose Gamma Irradiation on Some Phytochemicals and Scavenger Ability of in Vitro Culantro (Eryngium foetidum L.) Plantlets
نویسنده
چکیده
This research work aimed to enhance the concentration of some bioactive compounds in culantro (Eryngium foetidum L.) plantlets through gamma irradiation (0.0, 10.0, 20.0 and 40.0 Gy range) treatments. Gamma irradiation at 40.0 Gy significantly stimulated total phenolic, flavonoids, tannin and saponin contents. The effect of irradiation on antioxidant properties of culantro extracts was investigated by radical-scavenging effect on 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radicals and the content of thiobarbituric acid-reactive substances (TBARS). Some significant changes were observed in thiobarbituric acid-reactive substances between non-irradiated and irradiated samples. Irradiation at 40 Gy affected the DPPH radical-scavenging activity significantly. Total soluble sugars increased from 2.68 g/100 g dw at 0.0 Gy to 6.08 g/100 g dw at 10.0 Gy. Gamma radiation enhanced the level of ascorbic acid accumulation compared to control (non-irradiated) plantlets. Total chlorophyll decreased significantly under all irradiation treatments. These results suggested that both low -irradiation doses and tissue culture techniques could be used to produce culantro plantlets with high quantities of certain metabolites. _____________________________________________________________________________________________________________
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